Sperm survival during short-term storage and after cryopreservation of semen from striped trumpeter (Latris lineata)

Methods of short-term storage and cryopreservation were examined for semen from striped trumpeter (Laths lineata). For fresh semen at 18°C, the percentage of motile sperm declined rapidly from over 80% immediately after activation with sea water to less than 2% within 9 min after activation The motility after activation of undiluted fresh sperm stored at 5°C was maintained for two days and then declined markedly so that by the eighth day, sperm were mostly immotile after activation The post-thawing motility was higher for sperm frozen with a non-activating diluent containing 2 84 M DMSO in saline (117 mM NaCl) than in an activating glycerol (2 M) medium in dilute sea water (300 mOsm). Post-thawing motility was higher for a dilution rate of 1:5 (semen:diluent) than 1 2 or 1.11 but was similar when frozen semen was thawed at 10°, 20° or 30°C For semen stored at a range of volumes as pellets frozen on dry ice (0.2 to 2 0 mL) or straws frozen in liquid nitrogen vapor (0 25 to 0 5 mL) and thawed in a waterbath at 20°C, the post-thawing motilities were similar even though the patterns of cooling and thawing differed markedly between methods of freezing and sizes of pellets and straws. ©2000 by Elsevier Science In