Creation of bac genomic for cocoa (Theobroma cacao L.) [S04-15)

To develop genomic resources to study the structure and evolution of the perennial crop Theobroma cacao L. genome, we constructed a BAC library for the genotype Scavina 6 (Sca6). The BAC library contains 36864 clones with an average insert size of 120 Kbp which represent 10 haploid genome equivalents. Screening of the BAC library with a set of 14 genetically-anchored single copy probes identified an average of 9 BAC clones per probe, showing that all ten linkage groups are well represented in the BAC library. A set of resistance gene analogs (RGAs), previously mapped in cocoa, were used to identify RGA-containing BACs. This represent the first step to characherize identified QTLs for resistance to cocoa pathogens like Phtytophthora. Spp. (palmivora and megakarya). This library represents the first available BAC resource for structural genomic studies in cocoa and a valuable resource to physically characterize QTLs for agronomic traits but mainly for disease resistance and map-based cloning of resistance genes to major cocoa pathogens like Phytophthora ssp, Crinipelis perniciosa and Moniliophthora roreri. (Texte intégral