Influence of grafting technique on meristem micrografting of Douglas-fir

Three techniques for in vitro micrografting of shoot meristems from a 15-year-old clone of Douglas fir (#Pseudotsuga menziesii#) were compared. Although micrografting was feasible with all three procedures, the success rate was greatly influenced by the type of graft used. The best score (50% success) was obtained when using a miniaturized side-grafting technique. The micrografts, which could be easily acclimatized to in vivo conditions, exhibited however substantial variability with regard to scion development. (Résumé d'auteur