We have attempted to alleviate the pH dependency of triplex recognition of guanine by using intermolecular triplexes containing 2-amino-5-(2-deoxy-d-ribofuranosyl)pyridine (AP) as an analogue of 2'-deoxycytidine (dC). We find that for the beta-anomer of AP, the complex between (AP)6T6and the target site G6A6*T6C6is stable, generating a clear DNase I footprint at oligonucleotide concentrations as low as 0.25 microM at pH 5.0, in contrast to 50 microM C6T6which has no effect on the cleavage pattern. This complex is still stable at pH 6.5 producing a footprint with 1 microM oligonucleotide. Oligonucleotides containing the alpha-anomer of AP are much less effective than the beta-anomer, though in some instances they are more stable than the unm...
Homopurine sequences of duplex DNA are binding sites for triplex-forming oligodeoxyribopyrimidines. ...
Triple-helical nucleic acids are formed by binding an oligonucleotide within the major groove of dup...
DNase I footprinting has been used to study the formation of parallel triplexes at oligopurine targe...
We have achieved recognition of all 4 bp by triple helix formation at physiological pH, using triple...
We have used DNase I footprinting to examine DNA triple helix formation at a 12 base pair oligopurin...
AbstractWe have used DNase I footprinting to examine DNA triple helix formation at a 12 base pair ol...
Oligonucleotide-directed triple helix formation offers the possibility of acheiving sequence recogni...
The formation of intermolecular DNA triple helices offers the possibility of designing compounds wit...
DNase I footprinting has demonstrated that the thymidine analogues 5-propargylamino dU, 2'-aminoetho...
The deoxyribonucleoside 1-(2-deoxy-beta-D-ribofuranosyl)-3-methyl-5-amino-1H-pyrazolo4,3-d]py rimidi...
We have used DNase I footprinting, fluorescence and ultraviolet (UV) melting experiments and circula...
The deoxyribonucleoside 1-(2-deoxy-beta-D-ribofuranosyl)-3-methyl-5-amino-1H-pyrazolo4,3-d]py rimidi...
The deoxyribonucleoside 1-(2-deoxy-beta-D-ribofuranosyl)-3-methyl-5-amino-1H-pyrazolo4,3-d]py rimidi...
We have used DNase I footprinting, fluorescence and ultraviolet (UV) melting experiments and circula...
We have used DNase I footprinting, fluorescence and ultraviolet (UV) melting experiments and circula...
Homopurine sequences of duplex DNA are binding sites for triplex-forming oligodeoxyribopyrimidines. ...
Triple-helical nucleic acids are formed by binding an oligonucleotide within the major groove of dup...
DNase I footprinting has been used to study the formation of parallel triplexes at oligopurine targe...
We have achieved recognition of all 4 bp by triple helix formation at physiological pH, using triple...
We have used DNase I footprinting to examine DNA triple helix formation at a 12 base pair oligopurin...
AbstractWe have used DNase I footprinting to examine DNA triple helix formation at a 12 base pair ol...
Oligonucleotide-directed triple helix formation offers the possibility of acheiving sequence recogni...
The formation of intermolecular DNA triple helices offers the possibility of designing compounds wit...
DNase I footprinting has demonstrated that the thymidine analogues 5-propargylamino dU, 2'-aminoetho...
The deoxyribonucleoside 1-(2-deoxy-beta-D-ribofuranosyl)-3-methyl-5-amino-1H-pyrazolo4,3-d]py rimidi...
We have used DNase I footprinting, fluorescence and ultraviolet (UV) melting experiments and circula...
The deoxyribonucleoside 1-(2-deoxy-beta-D-ribofuranosyl)-3-methyl-5-amino-1H-pyrazolo4,3-d]py rimidi...
The deoxyribonucleoside 1-(2-deoxy-beta-D-ribofuranosyl)-3-methyl-5-amino-1H-pyrazolo4,3-d]py rimidi...
We have used DNase I footprinting, fluorescence and ultraviolet (UV) melting experiments and circula...
We have used DNase I footprinting, fluorescence and ultraviolet (UV) melting experiments and circula...
Homopurine sequences of duplex DNA are binding sites for triplex-forming oligodeoxyribopyrimidines. ...
Triple-helical nucleic acids are formed by binding an oligonucleotide within the major groove of dup...
DNase I footprinting has been used to study the formation of parallel triplexes at oligopurine targe...