Antioxidant efficacy of germinated seeds extracts of industrial hemp (Cannabis sativa L.)

Semena imajo pomembno vlogo v prehrani ljudi, nedavne študije pa so pokazale, da lahko kaljenje še izboljša njihovo hranilno vrednost. V diplomskem delu smo preiskovali antioksidativno učinkovitost industrijske konoplje (Cannabis sativa L.), uporabili smo izvlečke razmaščenih nekaljenih semen in kaljenih 24, 48, 72 ter 96 ur. Antioksidativno učinkovitost izvlečkov smo spremljali z metodo določitve sposobnosti lovljenja DPPH• radikala in metodo razbarvanja β-karotena. Osrednje rezultate smo podali kot delež preostalega DPPH• po 30 min oz. jih izrazili kot učinkovitost izvlečkov zoper razbarvanje β-karotena po 120 min. Ugotovili smo, da se sposobnost lovljenja DPPH• radikalov tekom kaljenja spreminja, pri čemer po 48 urah kaljenja zaznamo celo nižjo učinkovitost izvlečkov v primerjavi z nekaljenimi semeni (27 %). Najvišjo sposobnost lovljenja prostih radikalov imajo semena kaljena 96 ur (36 %). Pri metodi razbarvanja β-karotena rezultati nekoliko odstopajosprva sicer opazimo upad antioksidativne učinkovitosti. Za maksimalno (61 %) učinkovitost izvlečkov pa je zadostovalo že 72 ur kaljenja. Slaba korelacija obeh metod je potrdila razlike v antioksidativni učinkovitosti izvlečkov v homogenem in heterogenem okolju. Izkazalo se je, da se hitrost reakcije v obeh primerih s časom inkubacije upočasni, medtem ko se posamezni vzorci bistveno ne razlikujejo v kinetiki delovanja.Seeds have a very important role in human nutrition and recent studies have shown that germination of seeds improved their nutritional value. In graduate thesis we investigated antioxidant efficacy of industrial hemp (Cannabis sativa L.). The extracts of defatted non-germinated and germinated seeds for 24, 48, 72, 96 h were used. Antioxidant efficacy of extracts was measured with DPPH• radical scavenging method and β-carotene bleaching assay. The results were expressed as percentage of remaining DPPH• radical after 30 min and as extract efficiency against β-carotene bleaching after 120 min. From the DPPH• assay we can conclude that antioxidant efficacy is changing during germination. After 48 h lower extract efficacy is determined when compared to non-germinated seeds (27 %). The highest DPPH• scavenging activity express 96 h germinated seeds (36 %). These results don’t coincide with β-carotene bleaching testa decrease of antioxidant efficacy for extracts of seeds germinated for 24 h is noticed, meanwhile the highest extract efficacy (61 %) after 72 h of germination is determined. The weak correlation of both methods confirms differences in the antioxidative efficacy of extracts in a homogeneous and heterogeneous environment. It has been shown that the reaction rate decreases with time of incubation in both cases, while there is no differences in kinetics between individual samples