HPV genotype prevalence in Australian women undergoing routine cervical screening by cytology status prior to implementation of an HPV vaccination program

BACKGROUND: Data on the prevalence of cervical HPV genotypes in Australia by age and by grade of cytological abnormality are sparse. OBJECTIVE: Measure prevalence of HPV genotypes among 2620 Australian women by age and cytology status. STUDY DESIGN: Women presenting for routine Pap smear screening were recruited from diverse regions, including a significant sample of Indigenous women. DNA extracts prepared from Thinprep specimens were HPV genotyped by Roche LINEAR ARRAY HPV. RESULTS: HPV prevalence and genotype distribution were stratified by age (mean 32.6y) and Pap smear result (cytology normal in 86.7%). Overall HPV prevalence was 38.7% with high-risk HPV prevalence of 26.5%. Prevalence of HPV (66.3% in women40y), multiple HPV infection (45.5% in 40y) and vaccine-targeted genotypes (HPV 6/11/16/18) (34.1% in 40y) declined significantly with age. The six most common genotypes were: HPV 16 (8.3%), 51 (5.1%), 53 (4.7%), 62 (4.3%), 89 (3.9%) and 52 (3.8%). HR-HPV prevalence increased from 21.1% in women with normal cytology to 80.9% in those with cytologically predicted high-grade abnormalities (HGAs) (p