SINTESIS SENYAWA ASAM O-(4-METOKSI BENZOIL)-5-METILSALISILAT DAN UJI AKTIVITAS ANALGESIK PADA MENCIT (Mus musculus)

Background of the study: Synthesis of O- (4-methoxybenzoyl) -5-methylsalicylic acid compound from salicylic acid derivative had been performed. The O- (4-methoxybenzoyl) -5-methylsalicylic acid compound was expected to have higher analgesic activity than aspirin. Objective of the study: Produce an O- (4-methoxybenzoyl) -5-methalsalicylic acid compound of the reaction between 5-methylsalicylic acid and 4-methoxybenzoyl chloride reactor using an esterification method of acylhalide. Method: The synthesis was done using esterification method of acyl halide, while the analgesic activity test using the writhing test method. Result: New compounds which were produced from the synthesis were crystalline, white, odorless powder with a yield percentage of 34.3% and a melting point of 128.7 - 130.7oC. In TLC test, the results obtained 1 stain using 3 eluents: Ethylacetate: Chloroform (7: 3), Ethylacetate: Ethanol (7: 3), N-hexane: Ethylacetate: Methanol (4:4:2).Structure identification using the UV Spectrophotometer showed that the wavelengths were 206 nm and 258 nm. In the IR spectrophotometer, the wave number showed 3461,64 cm-1 (-OH); 2976,64 cm-1 (CH3); 1738,54; 1685,52 cm-1 (C = O); 1606,57 cm-1 (C = C); 1259.50 cm-1 (COOC). The HNMR spectrometer showed 14 H atoms. The activity test used three doses of 25 mg, 50 mg and 100 mg, each dose using as many as 6 mouse. The percentage of pain barrier were obtained at the dose of 25mg / kgBB = 35,94%, 50mg / kgBB = 54,60%, 100mg / kgBB = 63,78%. O-(O-4-methoxybenzyl) -5-methylsalicylic acid compound had ED50 of 46.54 mg / kgBB while the aspirin compound, which is a salicylic acid derivative, had ED50 of 135.68 mg / kgBB. Conclusion : The acid compounds of O-(4-methoxybenzoyl)- 5-methyl salicylate was successfully synthesized and had a higher activity than aspirin