Induction of Callus Cultures from the Leaves of Syzygium cumini(Linn.)Skeels in Woody Plant Medium with Variations of Growth Hormones

Syzygium cumini(Linn.)Skeels, known as jamblang,belongs to the family of guava(Myrtaceae).The plant can be found in India,Southeast Asia and Eastern Africa.This plant has a potential use for treatment of type2 diabetes mellitus.Although still visible,especially in rural areas,it is becoming very rare in big cities.Now a days,not many people use the fruit of this plant because of its scarcity.To over come the problem,in this research,jamblang plant was propagated in vitro using the leaves a sex plants to produce callus cultures in stead of the regeneration of whole plants.The sterilization was conducted by immersing them in the Mushi Guard solution(5mL/L),followed by the immersion in the solution of NaOCl.The callus cultures were here induced using WPM(Woody Plant Medium)with different concentrations of growth hormones,i.e.,naphthalene acetic acid(NAA)and benzyl aminopurine(BAP),in order to determine their optimum concentrations for the induction.There sults showed the optimum media of calluses induction was WPM with NAA 5ppm without any addition of BAP with an induction time of 30 days and percent age of callus cultures induction up to90%.The optimum media for calluses growth was also WPM with the addition of NAA 5 ppm.Their average growth index was 2.212 after the 30days of subculturing