Saliva sampling of alcoholic participants using three saliva collection methods

The potential of using saliva as a diagnostic fluid is well documented. The aim of this study was to assess the quality and quantity of saliva DNA of alcoholic and non-alcoholic participants using three saliva collection methods; DNA-SalTM (Oasis Diagnostics, USA), Oragene-DNA (DNA Genotek Inc, Ontario, Canada) and whole saliva collection method. Saliva DNA of non-alcoholic (n=30) and alcoholic participants (n=10) age between 25 and 35 years was assessed qualitatively and quantitatively using spectrophotometry. Saliva DNA quantity was the highest for all participants when using the DNA-Sal TM saliva collection kit (p<0.05). The use of a mechanical scraper provided only in the DNA-Sal TM kit may have contributed to the highest DNA yield for all participants. The quantity of saliva DNA when assessed using spectrophotometer was found to be significantly lower (p