Early Detection of Dengue Virus by Use of Reverse Transcription-Recombinase Polymerase Amplification
- Teoh, B.T.
- Sam, Sing Sin
- Tan, K.K.
- Danlami, M.B.
- Shu, M.H.
- Johari, Jefree
- Hooi, P.S.
- Brooks, D.
- Piepenburg, O.
- Nentwich, O.
- Wilder-Smith, A.
- Franco, L.
- Tenorio, A.
- AbuBakar, Sazaly
DOIAbstract
A method for the rapid diagnosis of early dengue virus (DENV) infection is highly needed. Here, a prototype reverse transcriptionrecombinase polymerase amplification (RT-RPA) assay was developed. The assay detected DENV RNA in <20 min without the need for thermocycling amplification. The assay enabled the detection of as few as 10 copies of DENV RNA. The designed RT-RPA primers and exo probe detected the DENV genome of at least 12 genotypes of DENV circulating globally without crossreacting with other arboviruses. We assessed the diagnostic performance of the RT-RPA assay for the detection of DENV RNA in 203 serum samples of patients with clinically suspected dengue. The sera were simultaneously tested for DENV using a reverse transcription...
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