Diet induced obesity is independent of metabolic endotoxemia and TLR4 signalling, but markedly increases hypothalamic expression of the acute phase protein, SerpinA3N

The authors gratefully acknowledge Doctoral Training Partnership funding from the BBSRC (M.J.D.), MRC (GA) and funding from the Scottish Government (P.J.M., A.W.R., A.W.W. and P.B.). The authors would like to acknowledge the support of the Maxwell compute cluster funded by the University of Aberdeen. We also thank the Centre for Genome-Enabled Biology and Medicine, University of Aberdeen for performing next-generation sequencing and RNAseq, Dr Sophie Shaw, for bioinformatic analysis of the RNAseq data, and Lynn Thomson for assistance with the TLR4−/− and CD14−/− mouse study, and Dr Richard Anderson and Dana Wilson for assistance with the long term high fat diet study. Supplementary information accompanies this paper at https://doi.org/10.1038/s41598-018-33928-4.Peer reviewedPublisher PD