Genetic and transgenic reagents for Drosophila simulans, D. mauritiana, D. yakuba, D. santomea, and D. virilis

Species of the Drosophila melanogaster species subgroup, including the species D. simulans, D. mauritiana, D. yakuba, and D. santomea, have long served as model systems for studying evolution. However, studies in these species have been limited by a paucity of genetic and transgenic reagents. Here, we describe a collection of transgenic and genetic strains generated to facilitate genetic studies within and between these species. We have generated many strains of each species containing mapped piggyBac transposons including an enhanced yellow fluorescent protein (EYFP) gene expressed in the eyes and a φC31 attP site-specific integration site. We have tested a subset of these lines for integration efficiency and reporter gene expression levels. We have also generated a smaller collection of other lines expressing other genetically encoded fluorescent molecules in the eyes and a number of other transgenic reagents that will be useful for functional studies in these species. In addition, we have mapped the insertion locations of 58 transposable elements in D. virilis that will be useful for genetic mapping studies.Fil: Stern, David L.. Janelia Research Campus; Estados UnidosFil: Crocker, Justin. Janelia Research Campus; Estados UnidosFil: Ding, Yun. Janelia Research Campus; Estados UnidosFil: Frankel, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Kappes, Gretchen. No especifica;Fil: Kim, Elizabeth. Janelia Research Campus; Estados UnidosFil: Kuzmickas, Ryan. Brigham and Women's Hospital; Estados UnidosFil: Lemire, Andrew. Janelia Research Campus; Estados UnidosFil: Mast, Joshua D.. California State University; Estados UnidosFil: Picard, Serge. University of Princeton; Estados Unido