Add to ORKGContains fulltext : 98485.pdf (publisher's version ) (Closed access)Traditionally, transgenic mice are generated by pronuclear injection of exogenous DNA. This technique has several limitations, including limited control over transgene expression, transgene cytotoxicity, -promiscuity and silencing, and founder mouse sterility. Here we describe two protocols to generate transgenic mice from ES cell clones carrying stably integrated exogenous DNA with inducible transgene expression
Our ability to decipher gene sequences has increased enormously with the advent of modern sequencing...
Transgenic techniques have prompted major contributions to a number of research areas of biology and...
This review highlights the use of transgenic mice and gene targeting in the study of reproduction, p...
Traditionally, transgenic mice are generated by pronuclear injection of exogenous DNA. This techniqu...
Genetic transformation of mice using pronuclear microinjection was demonstrated by a number of group...
This chapter describes the generation of transgenic mice and rats by microinjection of DNA construct...
Abstract Transgenic animals are extensively used to study in vivo gene function as well as to model ...
[[abstract]]Embryonic stem (ES) cells are considered to have potentials for tissue regeneration and ...
<p>A: Screen of transgenic founder mice by PCR. M: DL2000 DNA marker; 1–10: the founder mice derived...
[[abstract]]Embryonic stem (ES) cells are considered to have potentials for tissue regeneration and ...
8 p.-3 fig.-1 tab.We had previously developed a methodology to introduce foreign DNA into mouse eggs...
Abstract Background Transgenic mice have been used extensively to analyze gene function. Unfortunate...
Transgenic mice are increasingly used for gene function and regulation studies of mammalian genes. A...
Embryonic stem (ES) cells are considered to have potentials for tissue regeneration and treatment of...
Transgenic (Tg) animals are widely used in researching the characteristics of exogenous genes. Intra...
Our ability to decipher gene sequences has increased enormously with the advent of modern sequencing...
Transgenic techniques have prompted major contributions to a number of research areas of biology and...
This review highlights the use of transgenic mice and gene targeting in the study of reproduction, p...
Traditionally, transgenic mice are generated by pronuclear injection of exogenous DNA. This techniqu...
Genetic transformation of mice using pronuclear microinjection was demonstrated by a number of group...
This chapter describes the generation of transgenic mice and rats by microinjection of DNA construct...
Abstract Transgenic animals are extensively used to study in vivo gene function as well as to model ...
[[abstract]]Embryonic stem (ES) cells are considered to have potentials for tissue regeneration and ...
<p>A: Screen of transgenic founder mice by PCR. M: DL2000 DNA marker; 1–10: the founder mice derived...
[[abstract]]Embryonic stem (ES) cells are considered to have potentials for tissue regeneration and ...
8 p.-3 fig.-1 tab.We had previously developed a methodology to introduce foreign DNA into mouse eggs...
Abstract Background Transgenic mice have been used extensively to analyze gene function. Unfortunate...
Transgenic mice are increasingly used for gene function and regulation studies of mammalian genes. A...
Embryonic stem (ES) cells are considered to have potentials for tissue regeneration and treatment of...
Transgenic (Tg) animals are widely used in researching the characteristics of exogenous genes. Intra...
Our ability to decipher gene sequences has increased enormously with the advent of modern sequencing...
Transgenic techniques have prompted major contributions to a number of research areas of biology and...
This review highlights the use of transgenic mice and gene targeting in the study of reproduction, p...