Glutamine protects intestinal calcium absorption against oxidative stress and apoptosis
- Moine, Luciana Beatriz
- Díaz de Barboza, Gabriela Edith
- Pérez, Adriana del Carmen
- Benedetto, Maria Mercedes
- Tolosa, Nori Graciela
DOIAbstract
The aim of this study was to investigate whether glutamine (GLN) could block the inhibition of the intestinal Ca2+ absorption caused by menadione (MEN), and elucidate the underlying mechanisms. To do this, one-month old chicks were divided in four groups: 1) controls, 2) MEN treated, 3) GLN treated and 4) GLN treated before or after MEN treatment. Intestinal Ca2+ absorption as well as protein expression of molecules involved in the transcellular Ca2+ pathway were determined. Glutathione (GSH) and superoxide anion and activity of enzymes of the antioxidant system were evaluated. Apoptosis was measured by the TUNEL technique, the expression of FAS and FASL and the caspase-3 activity. A previous dose of 0.5 g GLN/kg of b.w. was necessary to sh...
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