Development of a nanostructured immunosensor for early and in situ detection of Xanthomonas arboricola in agricultural food production
- Regiart, Daniel Matias Gaston
- Rinaldi Tosi, Martin Edgardo
- Aranda, Pedro Rodolfo
- Bertolino, Franco Adrián
- Villarroel Rocha, Jhonny
- Sapag, Manuel Karim
- Messina, Germán Alejandro
- Raba, Julio
- Fernández Baldo, Martín Alejandro
DOIAbstract
We report a microfluidic electrochemical immunosensor for Xanthomonas arboricola (XA) determination, based on the covalently immobilization of monoclonal anti-XA antibody (anti-XA) on a previously amino functionalized SBA-15 in situ synthesized in the central channel of a glass-poly(dimethylsiloxane) microfluidic immunosensor. The synthetized amino-SBA-15 was characterized by N2 adsorption-desorption isotherm, scanning electron microscopy and infrared spectroscopy. XA was detected by a direct sandwich immunoassay through an alkaline phosphatase (AP) enzyme-labeled anti-XA conjugate. Later, the substrate p-aminophenyl phosphate was converted to p-aminophenol by AP. The enzymatic product was detected at +100 mV on a sputtered gold electrode. ...
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