In this study, wepropose an automatic procedure for digital image processing. Wedescribe a method that can efficiently quantify and characterizelipid droplets distributions in different cell types in culture.Prospectively, the lipid droplets detection method described in thiswork could be applied to static or time-lapse data, collected with asimple visible light or fluorescence microscopy equipment. Fullyautomated algorithms were implemented in Octave, a freely availablescientific package.Fil: Masone, Diego Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienci...
High-throughput screening of biologically active substances in cell cultures remains challenging des...
We report the application of supervised machine learning to the automated classification of lipid dr...
Abstract Adipogenesis is essential in in vitro experimentation to assess differentiation capability ...
In vitro differentiating adipocytes are sensitive to liquid manipulations and have the tendency to f...
The most recent discoveries in the biochemical field are highlighting the increasingly important rol...
There is increasing evidence showing that cytosolic lipid droplets, present in all eucaryotic cells,...
AbstractLipid droplets (LDs) are highly dynamic organelles that perform multiple functions, includin...
Human Adipose-derived mesenchymal stem/stromal cells (hASCs) are of great interest because of their ...
Lipid droplets (LDs) are the main storage organelles for triglycerides. Elucidation of lipid accumul...
There is increasing evidence showing that cytosolic lipid droplets, present in all eukaryotic cells,...
Quantitative characterization of a single-cell phenotype remains challenging. We combined a scalable...
Quantitative characterization of a single-cell phenotype remains challenging. We combined a scalable...
Lipid accumulation is largely investigated due to its role in many human diseases. The attention is ...
AbstractLipid droplets emerge as important intracellular organelles relevant for lipid homeostasis a...
<p>(A) BODIPY 493/503 stained LDs were counted in stacks of confocal laser scanning microscopy (CLSM...
High-throughput screening of biologically active substances in cell cultures remains challenging des...
We report the application of supervised machine learning to the automated classification of lipid dr...
Abstract Adipogenesis is essential in in vitro experimentation to assess differentiation capability ...
In vitro differentiating adipocytes are sensitive to liquid manipulations and have the tendency to f...
The most recent discoveries in the biochemical field are highlighting the increasingly important rol...
There is increasing evidence showing that cytosolic lipid droplets, present in all eucaryotic cells,...
AbstractLipid droplets (LDs) are highly dynamic organelles that perform multiple functions, includin...
Human Adipose-derived mesenchymal stem/stromal cells (hASCs) are of great interest because of their ...
Lipid droplets (LDs) are the main storage organelles for triglycerides. Elucidation of lipid accumul...
There is increasing evidence showing that cytosolic lipid droplets, present in all eukaryotic cells,...
Quantitative characterization of a single-cell phenotype remains challenging. We combined a scalable...
Quantitative characterization of a single-cell phenotype remains challenging. We combined a scalable...
Lipid accumulation is largely investigated due to its role in many human diseases. The attention is ...
AbstractLipid droplets emerge as important intracellular organelles relevant for lipid homeostasis a...
<p>(A) BODIPY 493/503 stained LDs were counted in stacks of confocal laser scanning microscopy (CLSM...
High-throughput screening of biologically active substances in cell cultures remains challenging des...
We report the application of supervised machine learning to the automated classification of lipid dr...
Abstract Adipogenesis is essential in in vitro experimentation to assess differentiation capability ...