Detection of Nitric Oxide and Determination of Nitrite Concentrations in Arabidopsis thaliana and Azospirilum brasilense
- Foresi, Noelia Pamela
- Correa Aragunde, Maria Natalia
- Amenta, Melina Beatriz
- Arruebarrena Di Palma, Andrés
- Creus, Cecilia Mónica
- Lamattina, Lorenzo
DOIAbstract
There is now general agreement that nitric oxide (NO) is an important and almost ubiquitous signal in plants. Nevertheless, there are still many controversial observations and different opinions on the importance and functions of NO in plants. Partly, this may be due to the difficulties in detecting and even more in quantifying NO. Here, we summarize protocols for detecting NO and quantifying nitrite concentration in Arabidopisis seedlings and for the NO real time measurement in biofilms formed by the plant growth promoting rhizobacteria Azospirillum brasilense (A. brasilense). NO in oxygen-containing aqueous solution has a short half-life that is often attributed to a rapid oxidation to nitrite. Here we detail the use of the fluorescent pr...
Add to ORKG