Simple, effective protocols have been developed for manual and machine‐assisted Boc‐chemistry solid phase peptide synthesis on polystyrene resins. These use in situ neutralization [i.e. neutralization simultaneous with coupling], high concentrations (> 0.2 M) of Boc‐amino acid‐OBt esters plus base for rapid coupling, 100% TFA for rapid Boc group removal, and a single short (30 s) DMF flow wash between deprotection/coupling and between coupling/deprotection. Single 10 min coupling times were used throughout. Overall cycle times were 15 min for manual and 19 min for machine‐assisted synthesis (75 residues per day). No racemization was detected in the base‐catalyzed coupling step. Several side reactions were studied, and eliminated. These incl...
In the solid-phase synthesis of BOC-Cys(CH2Ph)-Pro-Leu-Gly-NH2 (I, BOC = Me3CO2C), 1-hydrox...
Boc-resin-bound -hydroxy--amino-aldehydes are accessible starting from N-terminally bound amino acid...
The research done last semester was on solid-phase peptide synthesis. The technique of solid-phase p...
Simple, effective protocols have been developed for manual and machine-assisted Boc-chemistry solid ...
The chemical synthesis of peptides and small proteins is a powerful complementary strategy to recomb...
Solid-phase techniques have been introduced in the early sixties by Bruce Merrifield (Nobel Prize in...
Solid-phase synthesis, the most innovative and commonly used strategy for the chemical synthesis of ...
Solid phase peptide synthesis (SPSS) and Boc/Fmoc orthogonal protecting strategy promoted the rapid ...
Solid-phase peptide synthesis (SPPS) is the strategy of choice for the synthesis of peptides for res...
International audienceWe have developed a convenient method for the direct synthesis of peptide thio...
Copyright © 2013 Ekaterina F. Kolesanova et al.This is an open access article distributed under the ...
We have developed a new 4-alkoxybenzyl-derived linker that anchors the C-terminal amino acid to the ...
The work described here had as its goal the development of new techniques for very rapid synthesis o...
We have developed a convenient method for the direct synthesis of peptide thioesters, versatile inte...
1143-1147Solid phase peptide synthesis basically involves repetitive deblocking and coupling reactio...
In the solid-phase synthesis of BOC-Cys(CH2Ph)-Pro-Leu-Gly-NH2 (I, BOC = Me3CO2C), 1-hydrox...
Boc-resin-bound -hydroxy--amino-aldehydes are accessible starting from N-terminally bound amino acid...
The research done last semester was on solid-phase peptide synthesis. The technique of solid-phase p...
Simple, effective protocols have been developed for manual and machine-assisted Boc-chemistry solid ...
The chemical synthesis of peptides and small proteins is a powerful complementary strategy to recomb...
Solid-phase techniques have been introduced in the early sixties by Bruce Merrifield (Nobel Prize in...
Solid-phase synthesis, the most innovative and commonly used strategy for the chemical synthesis of ...
Solid phase peptide synthesis (SPSS) and Boc/Fmoc orthogonal protecting strategy promoted the rapid ...
Solid-phase peptide synthesis (SPPS) is the strategy of choice for the synthesis of peptides for res...
International audienceWe have developed a convenient method for the direct synthesis of peptide thio...
Copyright © 2013 Ekaterina F. Kolesanova et al.This is an open access article distributed under the ...
We have developed a new 4-alkoxybenzyl-derived linker that anchors the C-terminal amino acid to the ...
The work described here had as its goal the development of new techniques for very rapid synthesis o...
We have developed a convenient method for the direct synthesis of peptide thioesters, versatile inte...
1143-1147Solid phase peptide synthesis basically involves repetitive deblocking and coupling reactio...
In the solid-phase synthesis of BOC-Cys(CH2Ph)-Pro-Leu-Gly-NH2 (I, BOC = Me3CO2C), 1-hydrox...
Boc-resin-bound -hydroxy--amino-aldehydes are accessible starting from N-terminally bound amino acid...
The research done last semester was on solid-phase peptide synthesis. The technique of solid-phase p...