Endoplasmic reticulum stress induces 5-fluorouracil resistance in human colon cancer cells
- Kim, Joon Ki
- Kang, Kyoung Ah
- Piao, Mei Jing
- Ryu, Yea Seong
- Han, Xia
- Fernando, Pattage Madushan Dilhara Jayatissa
- Oh, Min Chang
- Park, Jeong Eon
- Shilnikova, Kristina
- Boo, Sun Jin
- Na, Soo-Young
- Jeong, Yong Joo
- Jeong, Seung Uk
- Hyun, Jin Won
Publication date
June 2016
DOI Publisher
Elsevier BV ISSN
1382-6689 Citation count (estimate)
6Abstract
Colon cancer can be treated with 5-fluorouracil (5-FU), but 5-FU resistance frequently occurs. We determined whether 5-FU resistance arises as a result of endoplasmic reticulum (ER) stress. 5-FU-resistant SNUC5 colon cancer cells (SNUC5/FUR cells) expressed higher levels of ER stress-related proteins than drug-sensitive SNUC5 cells. SNUC5/FUR cells also exhibited more intense ER staining and higher level of mitochondrial Ca2+ overload. SNUC5/FUR cells transfected with siRNA against GRP78, ATF6, ERK, or AKT were more sensitive to 5-FU than siControl RNA-transfected cells. These results suggested that 5-FU resistance was associated with ER stress in colon cancer. (C) 2016 Elsevier B.V. All rights reserved
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