Determination of chlorine dioxide on escherichia coli in drinking water

Pitje vode, onesnažene z bakterijo Escherichia coli (E. coli), lahko pripelje do okužbe, ta pa lahko povzroči resna bolezenska stanja. Osrednji namen diplomskega dela je bil ugotoviti učinkovitost klorovega dioksida pri uničenju bakterije E. coli v pitni vodi. V okviru diplomskega dela smo izvedli štiri eksperimente. Učinkovitost smo preverjali z uporabo 0,2 mg/l raztopine klorovega dioksida. Za raziskovanje smo uporabili pitno vodo iz mariborskega vodovoda, ki so jo za potrebe naših raziskav v Nacionalnem laboratoriju za zdravje, okolje in hrano Maribor (NLZOH) laboratorijsko »okužili« z E. coli. Št. bakterij prisotnih v vodi smo določevali s pomočjo metode membranske filtracije, koncentracijo skupnega klora v vodi pa s kolorimetrično metodo (DPD test). Ugotovili smo, da je klorov dioksid učinkovit pri uničenju (deaktivaciji) E. coli v pitni vodi. Rezultati kažejo, da je za deaktivacijo E. coli potreben kontaktni čas manjši od 1 min.The presence of Escherichia coli (E. coli) bacteria in drinking water is not very common, however, drinking water polluted with this bacteria can lead to infection and can cause serious illnesses. The main intention of this diploma was to figure out the effectiveness of chlorine dioxide in the inactivation of E. Coli bacteria from drinking water. We did four experiments. We checked the effectiveness of this process by using 0.2 mg/l of chlorine dioxide solution. For research we used drinking water from the Maribor water supply, which has been intentionally infected with E. coli by the Maribor National Laboratory for Health, Environment and Food (NLZOH). We determined the number of bacteria in the water with using membrane filter tehnique, and the total chlorine concentration by colorimetric method (DPD test). We concluded that chlorine dioxide is effective in killing (inactivating) E. coli in drinking water. The results show that E. coli cells were inactivated in less then 1 min