Comparative behavior of glucose oxidase and oxalate oxidase immobilized in mucin/chitosan hydrogels for biosensors applications
- Benavidez, Tomás Enrique
- Baruzzi, Ana Maria
DOIAbstract
The analytical response of amperometric glucose electrodes was explained in the framework of the properties of hydrogels with different muc/chit ratio at pH = 7.00 used as immobilization matrix for glucose oxidase. The continuous increase in the sensitivity of the electrodes with the amount of chitosan was attributed to a more efficient crosslinking without affecting the enzyme activity. The results were compared with those obtained using the same matrices to immobilize Oxalate Oxidase at pH = 2.85 where it has the highest activity. A maximum sensitivity at muc/chit 70/30 ratio for these oxalate electrodes was observed. Decrease in permeability of negatively charged oxalate with the chitosan content in the matrices, in spite of the high par...
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