Discovery of two novel β-glucosidases from an Amazon soil metagenomic library
- Bergmann, Jessica C.
- Costa, Ohana Yonara A.
- Gladden, John M.
- Singer, Steven
- Heins, Richard
- D'haeseleer, Patrik
- Simmons, Blake A.
- Quirino, Betania F.
DOIAbstract
An Amazon soil microbial community metagenomic fosmid library was functionally screened for β-glucosidase activity. Contig analysis of positive clones revealed the presence of two ORFs encoding novel β-glucosidases, AmBGL17 and AmBGL18, from the GH3 and GH1 families, respectively. Both AmBGL17 and AmBGL18 were functionally identified as β-glucosidases. The enzymatic activity of AmBGL17 was further characterized. AmBGL17 was tested with different substrates and showed highest activity on pNPβG substrate with an optimum temperature of 45 °C and an optimum pH of 6. AmBGL17 showed a V of 116 mM s and K of 0.30 ± 0.017 mM. This is the first report of β-glucosidases from an Amazon soil microbial community using a metagenomic approach
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