Study on cerebroprotective actions of Clerodendron glandulosumleaves extract against long term bilateral common carotid artery occlusion in rats

Stroke is a major cause of death and disability worldwide. The resulting burden on the society continues to grow, with increase in the incidence of stroke. Oxidative stress has been involved in the pathogenesis of several neurological diseases including acute stroke. Focal and global cerebral ischemia represents diseases that are common in the human population. In recent years much attention is being paid towards the exploration of herbal preparation, antioxidant agents and combination therapies including COX-2 inhibitors in experimental model of stroke. Possible effect of a hydroalcoholic leaf extract of Clerodendron glandulosumColeb (C. glandulosum) on oxidant-antioxidant status in ischemia-hypoperfusion injury in the rat forebrain has been investigated. Healthy adult male Wistar albino rats were divided into five groups (n = 8). Group I was served as Sham control (normal saline 1 ml/kg, orally), group II was served hypoperfusion control (normal saline 1 ml/kg, orally), group III, group IV were served as hydroalcoholic extract treated (200 and 400 mg/kg, orally) and group V was treated with Quercetin (10 mg/kg, orally) for 14 days to assess preventive and curative effects of C. glandulosum. Flavonoid and phenolic compounds exhibit a broad spectrum of biological activity, including antioxidant. C. glandulosum extract (200 and 400 mg/kg, p.o) was administered orally, once daily for a period of 2 weeks after the occlusion of BCCA. After 14th days rats were subjected to behavioral studies. After behavioral studies animals were sacrificed and brain was removed and homogenized. Estimation of Lipid peroxidation (LPO) Myeloperoxidase (MPO), estimation of protein levels and the activities of Superoxide dismutase (SOD), Catalase (CAT), were performed. Infarct size and histopathological changes were observed in treated groups. (C) 2016 Elsevier Masson SAS. All rights reserved