A single step solid phase radioimmunoassay (SS-SPRIA) has been developed for human chorionic,gonadotropin (hCG) using monoclonal antibodies (MAb) from culture media adsorbed immunochemically on plastic tubes. The assays have been found to be very simple in terms of operation and do not demand purification of MAbs. Several MAbs which do not show any displacement in liquid phase RIA and ELISA provide a satisfactory SS-SPRIA. Our investigations revealed that the assumption regarding the stability of the primary Mab-Ag complex during incubation and washing steps in ELISAs is not strictly valid for dissociable MAbs. A comparison of different assay systems suggests that the single step SPRIA offers additional advantages over conventionally used m...
A solid-phase sandwich enzyme immunoassay for human chorionic gonadotropin (hCG) employing monoclona...
A single-step solid-phase RIA (SS-SPRIA) developed in our laboratory using hybridoma culture superna...
We systematically screened a large panel of well-characterized monoclonal antibodies (mAb) directed ...
A single step solid phase radioimmunoassay (SS-SPRIA) has been developed for human chorionic,gonadot...
A measure of stability of a given epitope is an important parameter in the exploration of the utilit...
Two novel and simple methods have been developed for detecting monoclonal antibodies (MAbs) with ide...
Real-time kinetics of ligand-ligate interaction has predominantly been studied by either fluorescenc...
Monoclonal antibodies (MAbs) have been used extensively for identification of sequence-specific epit...
Kinetics of protein-protein or ligand-ligate interaction has predominantly been studied by optical s...
Proteins and peptides are comprised of both sequence-specific and conformation-specific epitopes. Se...
A method has been developed for biospecific interaction analysis between antigen and antibody using ...
A method for the determination of real-time kinetic parameters of antigen-antibody reaction using (1...
We have investigated the complex formation between an immobilized monoclonal antibody and antigens t...
A two-site sandwich enzyme immunoassay for human chrionic gonadotropin (hCG) employing monoclonal an...
Kinetic constants of MAb-hCG interactions have been determined using solid phase binding of I-125[hC...
A solid-phase sandwich enzyme immunoassay for human chorionic gonadotropin (hCG) employing monoclona...
A single-step solid-phase RIA (SS-SPRIA) developed in our laboratory using hybridoma culture superna...
We systematically screened a large panel of well-characterized monoclonal antibodies (mAb) directed ...
A single step solid phase radioimmunoassay (SS-SPRIA) has been developed for human chorionic,gonadot...
A measure of stability of a given epitope is an important parameter in the exploration of the utilit...
Two novel and simple methods have been developed for detecting monoclonal antibodies (MAbs) with ide...
Real-time kinetics of ligand-ligate interaction has predominantly been studied by either fluorescenc...
Monoclonal antibodies (MAbs) have been used extensively for identification of sequence-specific epit...
Kinetics of protein-protein or ligand-ligate interaction has predominantly been studied by optical s...
Proteins and peptides are comprised of both sequence-specific and conformation-specific epitopes. Se...
A method has been developed for biospecific interaction analysis between antigen and antibody using ...
A method for the determination of real-time kinetic parameters of antigen-antibody reaction using (1...
We have investigated the complex formation between an immobilized monoclonal antibody and antigens t...
A two-site sandwich enzyme immunoassay for human chrionic gonadotropin (hCG) employing monoclonal an...
Kinetic constants of MAb-hCG interactions have been determined using solid phase binding of I-125[hC...
A solid-phase sandwich enzyme immunoassay for human chorionic gonadotropin (hCG) employing monoclona...
A single-step solid-phase RIA (SS-SPRIA) developed in our laboratory using hybridoma culture superna...
We systematically screened a large panel of well-characterized monoclonal antibodies (mAb) directed ...