Assembly intermediates of icosahedral viruses are usually transient and are difficult to identify. In the present investigation, site-specific and deletion mutants of the coat protein gene of physalis mottle tymovirus (PhMV) were used to delineate the role of specific amino acid residues in the assembly of the virus and to identify intermediates in this process. N-terminal 30, 34, 35 and 39 amino acid deletion and single C-terminal (N188) deletion mutant proteins of PhMV were expressed in Escherichia coli. Site-specific mutants H69A, C75A, W96A, D144N, D144N-T151A, K143E and N188A were also constructed and expressed. The mutant protein lacking 30 amino acid residues from the N terminus self-assembled to T = 3 particles in vivo while deletio...
Physalis mottle tymovirus (previously named belladonna mottle virus, Iowa strain) RNA was cross-link...
The role of the 3' noncoding (NC) region of Physalis mottle tymovirus genomic RNA in the multiplicat...
Physalis mottle tymovirus (previously named belladonna mottle virus, Iowa strain) RNA was cross-link...
Assembly intermediates of icosahedral viruses are usually transient and are difficult to identify. I...
Assembly intermediates of icosahedral viruses are usually transient and are difficult to identify. I...
Virus-like particles (VLPs) serve as excellent model systems to identify the pathways of virus assem...
The coat protein gene of physalis mottle tymovirus (PhMV) was over expressed in Escherichia coli usi...
Virus-like particles (VLPs) serve as excellent model systems to identify the pathways of virus assem...
Physalis mottle tymovirus (PhMV) is a small spherical plant virus with its RNA genome encapsidated i...
Physalis mottle tymovirus (PhMV) is a small spherical plant virus with its RNA genome encapsidated i...
Viruses have served as excellent model systems for studies on the assembly/disassembly of macromolec...
The structure of the T=3 single stranded RNA tymovirus, physalis mottle virus (PhMV), has been deter...
AbstractStructural studies have implicated the TYMV N-terminal amino acids of the coat protein (CP) ...
Poliovirus mutants in neutralizing antigenic site 3B were constructed by replacing the glutamic acid...
Viral assembly is the process by which the viral genome becomes encapsidated by viral structural pro...
Physalis mottle tymovirus (previously named belladonna mottle virus, Iowa strain) RNA was cross-link...
The role of the 3' noncoding (NC) region of Physalis mottle tymovirus genomic RNA in the multiplicat...
Physalis mottle tymovirus (previously named belladonna mottle virus, Iowa strain) RNA was cross-link...
Assembly intermediates of icosahedral viruses are usually transient and are difficult to identify. I...
Assembly intermediates of icosahedral viruses are usually transient and are difficult to identify. I...
Virus-like particles (VLPs) serve as excellent model systems to identify the pathways of virus assem...
The coat protein gene of physalis mottle tymovirus (PhMV) was over expressed in Escherichia coli usi...
Virus-like particles (VLPs) serve as excellent model systems to identify the pathways of virus assem...
Physalis mottle tymovirus (PhMV) is a small spherical plant virus with its RNA genome encapsidated i...
Physalis mottle tymovirus (PhMV) is a small spherical plant virus with its RNA genome encapsidated i...
Viruses have served as excellent model systems for studies on the assembly/disassembly of macromolec...
The structure of the T=3 single stranded RNA tymovirus, physalis mottle virus (PhMV), has been deter...
AbstractStructural studies have implicated the TYMV N-terminal amino acids of the coat protein (CP) ...
Poliovirus mutants in neutralizing antigenic site 3B were constructed by replacing the glutamic acid...
Viral assembly is the process by which the viral genome becomes encapsidated by viral structural pro...
Physalis mottle tymovirus (previously named belladonna mottle virus, Iowa strain) RNA was cross-link...
The role of the 3' noncoding (NC) region of Physalis mottle tymovirus genomic RNA in the multiplicat...
Physalis mottle tymovirus (previously named belladonna mottle virus, Iowa strain) RNA was cross-link...