Antibodies to guanylic acid: Fractionation and specificities

Guanylic-acid-specific antibodies were elicited in rabbits) using as immunogen pG linked through $5^{\prime}$-phosphate to thyroglobulin. Specificity and affinity of antibodies to nucleotides, nucleosides, DNA, and RNA were studied by their binding to radioactive ligands and competition experiments. Guanylic-acid-specific antibodies do not bind to deoxyguanylic acid and have an average association constant of $10^7 M^{-1}$ at 4°C. Binding of the antibodies to $^3H-RNA$ is G-specifiC. The antibodies do not bind to $^{32}P-ssDNA$ or $^{32}P-dsDNA$. The pG-specific antibodies could be separated into different fractions by affinity chromatography. These fractions) though specific to pG) differ in their cross-reactivities to nucleosides and nucleotides