An improved inverse vegetation model has been designed to better specify both temperature and precipitation estimates from vegetation descriptions. It is based on the BIOME4 vegetation model and uses both vegetation δ¹³C and biome as constraints. Previous inverse models based on only one of the two proxies were already improvements over standard reconstruction methods such as the modern analog since these did not take into account some external forcings, for example CO₂ concentration. This new approach makes it possible to describe a potential "isotopic niche" defined by analogy with the "climatic niche" theory. Boreal and temperate biomes simulated by BIOME4 are considered in this study. We demonstrate the impact of CO₂ concentration on bi...