Carbon nanotubes non-covalently functionalized with cytochrome c: A new bioanalytical platform for building bienzymatic biosensors

Eguilaz Rubio, Marcos
Venegas, Constanza J.
Gutierrez, Alejandro
Rivas, Gustavo Adolfo
Bollo, Soledad

Open link

Publication date

September 2016

DOI

10.1016/j.microc.2016.04.018

Publisher

Elsevier BV

Journal

Microchemical Journal

Abstract

We report for the first time the use of glassy carbon electrodes (GCE) modified by drop-coating with multi-walled carbon nanotubes (MWCNTs) dispersed in cytochrome c as platform for the construction of bienzymatic biosensors, using the glycoenzyme glucose oxidase (GOx) as model. The sensitivity of the resulting biosensor at -0.050 V towards glucose using hydroquinone (H2Q) as redox mediator was (96 ± 2) μA M-1, with a linear range between 1.00 × 10-4 M and 1.00 × 10-3 M and a detection limit of 8 μM. The biosensor demonstrated to be highly reproducible with RSD. values of 6.1% for five different bioelectrodes. The combination of the excellent dispersing properties of Cyt c, the stability of MWCNTs-Cyt c dispersion and the intimate interacti...