CREB3L1-mediated functional and structural adaptation of the secretory pathway in hormone-stimulated thyroid cells
- García, Iris Alejandra
- Torres Demichelis, Vanina Andrea
- Viale, Diego Luis
- Di Giusto, Pablo
- Ezhova, Yulia
- Polishchuk, Roman S.
- Sampieri, Luciana
- Martinez, Hernán
- Sztul, Elizabeth
- Alvarez, Cecilia Ines
DOIAbstract
Many secretory cells increase the synthesis and secretion of cargo proteins in response to specific stimuli. How cells couple increased cargo load with a coordinate rise in secretory capacity to ensure efficient transport is not well understood. We used thyroid cells stimulated with thyrotropin (TSH) to demonstrate a coordinate increase in the production of thyroid-specific cargo proteins and ER-Golgi transport factors, and a parallel expansion of the Golgi complex. TSH also increased expression of the CREB3L1 transcription factor, which alone caused amplified transport factor levels and Golgi enlargement. Furthermore, CREB3L1 potentiated the TSH-induced increase in Golgi volume. A dominant-negative CREB3L1 construct hampered the ability of...
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