The effect of the thermospheric vertical neutral wind on vertical total electron content (vTEC) variations including longitudinal anomaly, remaining winter anomaly, mid-latitude summer night anomaly, and semiannual anomaly is studied at mid-latitude regions around zero magnetic declination at midnight during high solar activity. By using the principal component analysis (PCA) numerical technique, this work studies the spatial and temporal variations of the ionosphere at midnight over mid-latitude regions during 2000–2002. PCA is applied to a time series of global vTEC maps produced by the International Global Navigation Satellite System (GNSS) Service. Four regions were studied in particular, each located at mid-latitude and approximately c...