Epigenetic activation of Sox2 gene in the developing vertebrate neural plate
- Bouzas, Santiago Oscar
- Marini, Melisa Soledad
- Torres Zelada, Eliana
- Buzzi, Ailín Leticia
- Morales Vicente, David A.
- Strobl Mazulla, Pablo Hernan
DOIAbstract
One of the earliest manifestations of neural induction is onset of expression of the neural marker Sox2, mediated by the activation of the enhancers N1 and N2. By using loss and gain of function, we find that Sox2 expression requires the activity of JmjD2A and the Msk1 kinase, which can respectively demethylate the repressive H3K9me3 mark and phosphorylate the activating H3S10 (H3S10ph) mark. Bimolecular fluorescence complementation reveals that the adaptor protein 14-3-3, known to bind to H3S10ph, interacts with JMJD2A and may be involved in its recruitment to regulatory regions of the Sox2 gene. Chromatin immunoprecipitation reveals dynamic binding of JMJD2A to the Sox2 promoter and N-1 enhancer at the time of neural plate induction. Fina...
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