Selective Impact of HIV Disease Progression on the Innate Immune System in the Human Female Reproductive Tract
- Lahey Timothy
- Ghosh Mimi
- Fahey John V.
- Shen Zheng
- Mukura Lucy R.
- Song Yan
- Cu-Uvin Susan
- Mayer Kenneth H.
- Wright Peter F.
- Kappes John C.
- Ochsenbauer Christina
- Wira Charles R.
DOIAbstract
We have previously demonstrated intrinsic anti-HIV activity in cervicovaginal lavage (CVL) from HIV-infected women with high CD4 counts and not on antiretroviral therapy. However, the impact of HIV disease progression on CVL innate immune responses has not been delineated.CVL from 57 HIV-infected women not on antiretroviral therapy were collected by washing the cervicovaginal area with 10 ml of sterile normal saline. We characterized subject HIV disease progression by CD4 count strata: >500 cells/µl, 200–500 cells/µl, or <200 cells/µl of blood. To assess CVL anti-HIV activity, we incubated TZM-bl cells with HIV plus or minus CVL. Antimicrobials, cytokines, chemokines and anti-gp160 HIV IgG antibodies were measured by ELISA and Luminex.CVL e...
Add to ORKG