Quantitative real-time PCR (qPCR) is a widely used technique for gene expression analysis. Its reliability is highly dependent upon selection of the appropriate reference genes for accurate gene expression normalization. In this study, we investigated the expression stability of 10 commonly used reference genes in a mouse myocardial infarction model.The expression stability of the 10 reference genes (Actb, B2m, Eef1a1, Gapdh, Hprt, Polr2a, Ppia, Rpl13a, Tbp, Tpt1) was analyzed using the geNorm software. Overall, the combination of Hprt, Rpl13a and Tpt1 was the most stable reference gene set in our experiments. Gapdh, Polr2a and Actb consistently showed the highest gene expression variability and the expression levels of Gapdh, Polr2a, Actb,...
The study of uterine gene expression patterns is valuable for understanding the biological and molec...
In biological research the analysis of gene expression levels in cells and tissues can be a powerful...
<div><p>The ability to obtain accurate and reproducible data using quantitative real-time Polymerase...
Quantitative real-time PCR (qPCR) is a widely used technique for gene expression analysis. Its relia...
BACKGROUND: Quantitative real-time PCR (qPCR) is a widely used technique for gene expression analysi...
Background Quantitative real-time RT-PCR (RT-qPCR) is a highly sensitive method for ...
To be accurate, quantitative Polymerase Chain Reaction (qPCR) studies require a set of stable refere...
Abstract Background Real-time quantitative polymerase chain reaction (RT-qPCR) is a widely-used stan...
Abstract Background Real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR)...
NF. Every bar represents change in normalization accuracy when stepwise adding more endogenous contr...
Aims Quantitative real-time RT-PCR (RT-qPCR) has become the method of choice for mRNA quantification...
Quantitative real time polymerase chain reaction (qPCR) has become a widely used tool to examine gen...
<div><p>The choice of reference genes that are stably expressed amongst treatment groups is a crucia...
The study of uterine gene expression patterns is valuable for understanding the biological and molec...
Proper data normalization in quantitative real-time reverse-transcription polymerase chain reaction ...
The study of uterine gene expression patterns is valuable for understanding the biological and molec...
In biological research the analysis of gene expression levels in cells and tissues can be a powerful...
<div><p>The ability to obtain accurate and reproducible data using quantitative real-time Polymerase...
Quantitative real-time PCR (qPCR) is a widely used technique for gene expression analysis. Its relia...
BACKGROUND: Quantitative real-time PCR (qPCR) is a widely used technique for gene expression analysi...
Background Quantitative real-time RT-PCR (RT-qPCR) is a highly sensitive method for ...
To be accurate, quantitative Polymerase Chain Reaction (qPCR) studies require a set of stable refere...
Abstract Background Real-time quantitative polymerase chain reaction (RT-qPCR) is a widely-used stan...
Abstract Background Real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR)...
NF. Every bar represents change in normalization accuracy when stepwise adding more endogenous contr...
Aims Quantitative real-time RT-PCR (RT-qPCR) has become the method of choice for mRNA quantification...
Quantitative real time polymerase chain reaction (qPCR) has become a widely used tool to examine gen...
<div><p>The choice of reference genes that are stably expressed amongst treatment groups is a crucia...
The study of uterine gene expression patterns is valuable for understanding the biological and molec...
Proper data normalization in quantitative real-time reverse-transcription polymerase chain reaction ...
The study of uterine gene expression patterns is valuable for understanding the biological and molec...
In biological research the analysis of gene expression levels in cells and tissues can be a powerful...
<div><p>The ability to obtain accurate and reproducible data using quantitative real-time Polymerase...