Comparasion of sperm viability stainings and fixations

The research applied viability staining techniques, originally described for frozen bull spermatozoa. The objective of this study was to compare three different methods of staining with different concentration of formalin in order to evaluate frozen bull sperm viability. Frozen bull sperm samples were stained with 0.1% Congo red, 0.16% Chicago sky blue and 0.2% trypan blue with the concentrations of 0 (control), 0.1% and 0.3% formalin fixative treatment to each staining techniques to compare the staining and percentage of fixative for the viability of bull sperm. 0.16% CSB staining method replaces both 0.2% TB and 0.1% CR which was true for those findings to replace 0.2% TB staining with CSB. As compared to the three staining methods 0.16% Chicago sky blue with 0.3% of formalin was the highest mean of sperm viable counted while 0.1% Congo red staining showed the lowest with the same concentration of formalin in the PBS.MscCandidate of Animal Science M.S