Functional analysis of a prostate cancer risk enhancer at 7p15.2

2018-07-26Prostate cancer (PCa) is the second most commonly diagnosed cancer in men in the United States and one of the most common types of cancer in men worldwide. To better understand the gene regulation mechanisms underpinning prostate cancer progression, a PCa risk locus was deleted using clustered regularly interspaced short palindromic repeats (CRISPR) in two prostate cell lines, with and without an active enhancer mark encompassing the risk SNP within the locus. RNA-seq, colony formation assays, and cell proliferation assays were performed to investigate the transcriptomic and phenotypic changes. My results suggest that this locus does affect prostate cancer susceptibility