A molecular phylogeny of the tribe Ochthebiini (Coleoptera, Hydraenidae, Ochthebiinae)

This is the pre-peer reviewed version of the following article: Villastrigo, Adrián; Jäch, Manfred A.; Cardoso, Anabela ; Valladares, Luis F.; Ribera, Ignacio; 2018; "A molecular phylogeny of the tribe Ochthebiini (Coleoptera, Hydraenidae, Ochthebiinae)"; Systematic Entomology, which has been published in final form at https://doi.org/10.1111/syen.12318.This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.El post print de este trabajo se encuentra en acceso abierto en http://hdl.handle.net/10261/168475.Postprint article is available in open acces at http://hdl.handle.net/10261/168475Ochthebiinae, with ca. 650 species distributed worldwide, are the second most speciose subfamily of the aquatic beetle family Hydraenidae. They are the ecologically most diverse subfamily, with terrestrial species as well as species in almost all types of aquatic habitats, including hypersaline waters. Ochthebiinae include the tribes Ochtheosini (four species in three genera) and Ochthebiini. We provide here the first comprehensive phylogeny of the tribe Ochthebiini, based on 188 species and four subspecies from most genera, subgenera and species groups. We obtained sequence data for a combination of mitochondrial and nuclear gene fragments including the 5’ and 3’ ends of the cytochrome c oxidase subunit 1, the 5’ end of 16S RNA plus the Leucine tRNA transfer plus 5’ end of NADH dehydrogenase subunit I, and internal fragments of the large and small ribosomal units. The analyses with maximum likelihood and Bayesian probabilities consistently recovered a generally well supported phylogeny, with most currently accepted taxa and species groups as monophyletic. We provide a new classification of the tribe based on our phylogenetic results, with three genera: Meropathus Enderlein, Ochthebius Leach and Protochthebius Perkins. Meropathus in its new sense includes five subgenera in addition to Meropathus s.str.: (1) M. (Hygrotympanogaster) Perkins comb.n.; (2) M. (Plesiotympanogaster) Perkins comb.n.; (3) M. (Topotympanogaster) Perkins comb.n. (the last three transferred from Tympanogaster); (4) M. (Tympallopatrum) Perkins comb.n.; and (5) M. (Tympanogaster) Janssens comb.n. (both currently considered as genera). The genus Ochthebius is here divided into nine subgenera in addition to Ochthebius s.str.: (1) O. (Angiochthebius) Jäch & Ribera; (2) O. (Asiobates) Thomson; (3) O. (Aulacochthebius) Kuwert; (4) O. (Cobalius) Rey; (5) O. (Enicocerus) Stephens; (6) O. (Gymnanthelius) Perkins comb.n.; (7) O. (Gymnochthebius) Orchymont comb.n.; (8) O. (Hughleechia) Perkins comb.n.; and (9) O. (Micragasma) Sahlberg. Within Ochthebius s.str., 17 species groups are proposed, five of them newly established (3, 9, 11, 13 and 16): (1) andraei; (2) atriceps; (3) corrugatus; (4) foveolatus; (5) kosiensis; (6) lobicollis; (7) marinus; (8) metallescens; (9) nitidipennis; (10) notabilis; (11) peisonis; (12) punctatus; (13) quadricollis; (14) rivalis; (15) strigosus; (16) sumatrensis; and (17) vandykei. We could not obtain sequences of any species of Protochthebius, which is therefore maintained as a distinct genus of Ochthebiini. We elevated to species rank two subspecies of Ochthebius: O. fallaciosus Ganglbauer stat.n. (former subspecies of O. viridis Peyron) and O. deletus Rey stat.rest. (former subspecies of O. subpictus Wollaston).A.V. has a FPI-MINECO PhD grant from the Spanish Government. This work has been partly funded by project CGL2013-48950-C2 (AEI/FEDER, UE) to I.R.N