Production and Characterization of Human-Human Hybridoma Autoantibodies from Patients with Systemic Lupus Erythematosus

Note:La maladie lupique (SLE) se caractérise par la production d'autoanticorps et par la présence de diverses anomalies biologiques, d'origine inconnue. L'étude de la diversité de ces autoanticorps fut entreprise à l'aide de la production d'anticorps monoclonaux et anti-idiotypiques. Le meilleur rendement en hybridomes (7.5%) et en anticorps anti-DNA (17%) fut obtenu par l'emploi d'un rapport cellulaire 1:1 et d'une densité cellulaire de 4x105 cellules/ml. L'étude de la capacité de liaison antigénique des anticorps monoclonaux a révélé que 70% d'entre eux étaient polyspécifiques et que 55% de ceux-ci avaient en plus une activité anti-IgG (RF) et/ou anti-coagulante (PTT). […]The origin of autoantibodies, as well as the mechanisms which account for the diversity of serological abnormalities in SLE, is unknown. The antibody diversity in SLE was analyzed here by producing hybridoma antibodies and raising anti-idiotype antisera to these hybridoma antibodies. The optimal yield of 7.5% hybridomas and 17% anti-DNA antibodies occurred with the use of a PBL to GM 4672 cell ratio of 1:1 and a cell plating density of 4x105 cells/m!. Eighteen percent of all hybridoma antibodies produced had an SLE-related antibody activity and all were of the IgM class. Each antibody had its own pattern of reactivity: 69% had multiple antigen-binding specificities and 55% of these had in addition RF and/or PTT activity. […