Relationship between Zrc1, zincosomes and zinc tolerance.

<p>(A) Cells were challenged with potentially toxic zinc (1 mM), stained with zinquin and fluorescence determined. <i>P</i> < 0.0001 compared to wild type and revertant. (B) Micrographs of cells treated as in A. Note that <i>zrc1</i>Δ is highly defective for zincosome formation in response to 1 mM ZnSO₄ –a condition under which wild type, but not <i>zrc1</i>Δ cells can grow (<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1007013#ppat.1007013.s008" target="_blank">S7 Fig</a>).