Ank1 and Ank6 promote p65 removal from the nucleus in an exportin 1-dependent manner.

<p>HeLa cells were transfected to express Flag-tagged BAP, Ank1, Ank6, or IκBα SR. At 16 h, the cells were treated with LMB or vehicle control for 1 h. The media was replaced with media containing TNFα or vehicle for 30 min. The cells were then fixed, screened with antibodies specific for the Flag epitope and p65, and examined by confocal microscopy. Representative fluorescence images are presented in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1007023#ppat.1007023.s007" target="_blank">S7 Fig</a>. The mean percentage <u>+</u> SD of cells exhibiting p65 in the nucleus was determined. Quadruplicate samples of 100 cells each were counted per time point. Statistically significant (*<i>P</i> < 0.05; ***<i>P</i> < 0.001) values are indicated. n.s., not significant. Results are representative of three independent experiments.