Enhanced T_FH and GC B cell responses in T cell-specific <i>Cd2ap</i>-deficient mice in response to acute viral infection.

<p>(A) Flow cytometric analysis of expression of CD4, CD8 and CD44 and binding of H-2D^b(gp33-41) tetramer in splenocytes 8 days after LCMV-Armstrong infection. Total CD8 T cells and LCMV-gp33-specific CD8 T cells are shown with rectangular gates. (B) Expression of CD8 and KLRG1 in splenocytes 8 days after LCMV-Armstrong infection. (C) Expression of CD44 and binding of I-A^b(gp66-77) tetramer in CD4⁺ splenic T cells 8 days after LCMV-Armstrong infection. LCMV-gp66-specific CD4 T cells are shown with rectangular gates. (E, G) Expression of PD-1, CXCR5 and Ly6C and binding of I-A^b (gp66-77)⁺ tetramer of CD4⁺ T cells and Fas and GL7 expression in B cells in the spleens of <i>Cd4</i>-cre⁺ <i>Cd2ap</i>^F/F and control <i>Cd2ap</i>^F/F mice 8 (E) and 22 (G) days after infection. T_FH cells and GC B cells are shown with rectangular gates. Representative plots are shown with percentages of gated cells. Statistical analyses from 4–6 mice in 2 independent experiments are shown with means and standard deviation in (D, F, H).