CpdA does not support GR nuclear accumulation yet induces HSP70 gene expression in MM1.S cells.

<p>(A) MM1.S cells were treated for 2h with Dex (10^-6M) or CpdA (5.10^-6M) and Nucleus (N)–Cytoplasm (C) fractionation was performed. Protein lysates were prepared and WB analysis was performed, detecting the protein levels of GR (90-95kDa) and PARP (89 and 113 kDa, N fraction control), with tubulin (55kDa, C fraction control) serving as loading control. WB results are representative of 2 independent experiments. (B) MM1.S cells were treated for 6h with CpdA (10^-5M). RNA was isolated and subjected to RT-QPCR, detecting the mRNA levels of <i>HSPA1A</i> with <i>SDHA</i>, <i>YWHAZ</i> and <i>RPL13A</i> serving as reference genes. The bar plot represents the mean +/- SEM of 5 biological replicates. A two-tailed unpaired t-test was performed on log transformed data using GraphPad Prism 7. *** = <i>P</i> < 0.001.