Resistance to 5-FC, 6-azauracil and 8-azaguanine conferred by overexpression of different genes.

<p>The genes isolated in our HAP resistance screen were tested for resistance to 5-FC, 6-AzaU and 8-AzaG. No drug controls are also shown. Also included were the genes isolated in our previous screen for 5-FU resistance [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0196840#pone.0196840.ref016" target="_blank">16</a>]. Cells transformed with the empty vector pHR81 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0196840#pone.0196840.ref030" target="_blank">30</a>] were included as a negative control. Transformants were grown in liquid medium to late exponential phase, serially diluted, and then spotted onto uracil- and adenine-less SC galactose (Gal-UA) plates with or without HAP, 5-FU, 5-FC, 6-AzaU or 8-AzaG at the indicated concentrations. An empty row (Empty) was left below the pADE4 and pCPA1 transformants in order to prevent effects on adjacent strains due to the release of adenine and uracil into the media.