Design, Synthesis, Antiviral Bioactivity, and Defense Mechanisms of Novel Dithioacetal Derivatives Bearing a Strobilurin Moiety

A series of dithioacetal derivatives bearing a strobilurin moiety were designed and synthesized on the basis of our previous work. The antiviral activities of these compounds against Potato virus Y (PVY), Cucumber mosaic virus (CMV), and Tobacco mosaic virus (TMV) were systematically evaluated. Bioassay results indicated that <b>C14</b> elicited excellent curative and protective activities against PVY, CMV, and TMV. The former had 50% effective concentrations (EC₅₀) of 125.3, 108.9, and 181.7 μg/mL, respectively, and the latter had 148.4, 113.2, and 214.6 μg/mL, respectively, which were significantly superior to those of lead compound <b>6f</b> (297.6, 259.6, and 582.4 μg/mL and 281.5, 244.3, and 546.3 μg/mL, respectively), Ningnanmycin (440.5, 549.1, and 373.8 μg/mL and 425.3, 513.3, and 242.7 μg/mL, respectively), Chitosan oligosaccharide (553.4, 582.8, and 513.8 μg/mL and 547.3, 570.6, and 507.9 μg/mL, respectively), and Ribavirin (677.4, 690.3, and 686.5 μg/mL and 652.7, 665.4, and 653.4 μg/mL, respectively). Moreover, defensive enzyme activities and RT-qPCR analysis demonstrated that the antiviral activity was associated with the changes of SOD, CAT, and POD activities in tobacco, which was proved by the related proteins of abscisic acid signaling pathway. This work provided a basis for further design, structural modification, and development of dithioacetal derivatives as new antiviral agents