Additional file 1: of Outgrowth of Rice Tillers Requires Availability of Glutamine in the Basal Portions of Shoots

Figure S1. qPCR analysis of OsGS1;1 and OsGS1;2 in the basal portions of wild-type shoots using MSX. Wild-type rice seedlings were grown hydroponically in the presence of 1 mM NH4Cl until the fourth-leaf stage and then in water for 3 d. After pre-treatment with 1 mM MSX for 2 h, the seedlings were treated for 8 h with 1 mM NH4+ (MSX + NH4+) or 5 mM glutamine (MSX + Gln). The seedlings without MSX pre-treatment were also treated for 8 h with 1 mM NH4+ (+NH4+), 5 mM glutamine (Gln) or without nitrogen nutrients (-N). qPCR analysis of OsGS1;1 (a) and OsGS1;2 (b) were performed in the shoot basal parts. Fold changes of transcript levels in each treatment relative to those in –N were calculated, and mean values with SE of four independent samples are shown. Asterisks denote statistically significant differences between samples treated without 1 mM NH4+ and each treated sample (*, P < 0.05 by Student’s t-test). (EPS 1807 kb