Correlation between the neutrophil score, LDGs, and neutrophil activation.

<p><b>(A)</b> Flow plots showing the strategy for gating LDGs. PBMCs were isolated over a Ficoll gradient and then the CD10⁺CD15⁺ granulocytes gated as shown. As shown in the panels on the right, these cells were CD14^loCD16⁺ and had a unique forward (FSC) and side (SSC) scatter profile, consistent with the reported LDG phenotype [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0196117#pone.0196117.ref024" target="_blank">24</a>]. <b>(B)</b> Correlation between the neutrophil score and the number of LDGs per ml or neutrophil count. <b>(C)</b> Flow plot showing the region used to gate activated (CD11b^hiCD66b^hi) cells within the whole peripheral blood CD10⁺CD15⁺ neutrophil population. <b>(D)</b> Correlation between the neutrophil score, the number of activated neutrophils or LDGs in the peripheral blood.