Protein glycosylation is a common protein post-translational modification (PTM) in living organisms and has been shown to associate with multiple diseases, and thus may potentially be a biomarker of such diseases. Efficient protein/glycoprotein extraction is a crucial step in the preparation of <i>N</i>-glycans derived from glycoproteins prior to LC-MS analysis. Convenient, efficient and unbiased sample preparation protocols are needed. Herein, we evaluated the use of sodium deoxycholate (SDC) acidic labile detergent to release <i>N</i>-glycans of glycoproteins derived from biological samples such as cancer cell lines. Compared to the filter-aided sample preparation approach, the sodium deoxycholate (SDC) assisted approach was determined to...
Glycosylation is one of the most prevalent post-translational modifications found on biomolecules th...
<div><p>Glycan analysis may result in exploitation of glycan biomarkers and evaluation of heterogene...
Glycoprotein glycosylation is altered in many diseases, including congenital disorders of glycosylat...
Protein glycosylation is a common protein post-translational modification (PTM) in living organisms ...
Glycosylation is one of the most common forms of post-translational modifications on proteins, and g...
The glycosylation of proteins is responsible for their structural and functional roles in many cellu...
Glycosylation is one of the most common protein modifications and profoundly regulates many biologic...
A method has been developed for release/isolation of O-glycans from glycoproteins in whole cell lysa...
Glycosylation is an important post-translational modification of proteins. Many diseases, such as ca...
Changes in glycosylation signatures of cells have been associated with pathological processes in can...
The glycosylation of biologics is an important factor in pharmacological functions such as efficacy,...
Because routine preparation of glycan samples involves multiple reaction and cleaning steps at which...
A method has been developed for release/isolation of O-glycans from glycoproteins in whole cell lysa...
The N-glycan diversity of human serum glycoproteins, i.e., the human blood serum N-glycome, is both ...
The sensitive and specific detection of glycans via mass spectrometry (MS) remains a significant cha...
Glycosylation is one of the most prevalent post-translational modifications found on biomolecules th...
<div><p>Glycan analysis may result in exploitation of glycan biomarkers and evaluation of heterogene...
Glycoprotein glycosylation is altered in many diseases, including congenital disorders of glycosylat...
Protein glycosylation is a common protein post-translational modification (PTM) in living organisms ...
Glycosylation is one of the most common forms of post-translational modifications on proteins, and g...
The glycosylation of proteins is responsible for their structural and functional roles in many cellu...
Glycosylation is one of the most common protein modifications and profoundly regulates many biologic...
A method has been developed for release/isolation of O-glycans from glycoproteins in whole cell lysa...
Glycosylation is an important post-translational modification of proteins. Many diseases, such as ca...
Changes in glycosylation signatures of cells have been associated with pathological processes in can...
The glycosylation of biologics is an important factor in pharmacological functions such as efficacy,...
Because routine preparation of glycan samples involves multiple reaction and cleaning steps at which...
A method has been developed for release/isolation of O-glycans from glycoproteins in whole cell lysa...
The N-glycan diversity of human serum glycoproteins, i.e., the human blood serum N-glycome, is both ...
The sensitive and specific detection of glycans via mass spectrometry (MS) remains a significant cha...
Glycosylation is one of the most prevalent post-translational modifications found on biomolecules th...
<div><p>Glycan analysis may result in exploitation of glycan biomarkers and evaluation of heterogene...
Glycoprotein glycosylation is altered in many diseases, including congenital disorders of glycosylat...