Visualization of cross-links in GLA-trimer.
- Torben Schiffner (671875)
- Jesper Pallesen (5204072)
- Rebecca A. Russell (265956)
- Jonathan Dodd (4344523)
- Natalia de Val (460714)
- Celia C. LaBranche (172138)
- David Montefiori (161606)
- Georgia D. Tomaras (164058)
- Xiaoying Shen (164029)
- Scarlett L. Harris (5204075)
- Amin E. Moghaddam (5204069)
- Oleksandr Kalyuzhniy (3075177)
- Rogier W. Sanders (260873)
- Laura E. McCoy (386535)
- John P. Moore (260880)
- Andrew B. Ward (409527)
- Quentin J. Sattentau (207789)
DOIAbstract
<p>(<b>a</b>) Top view of the GLA-SOSIP trimer-PGV04 Fab complex with lysines (K)169 and arginines (R)166 displayed in green. Extra density corresponding to GLA crosslinks between K and R side chains is colored in yellow. (<b>b</b>) Distances between K169 and R166 side chains in the GLA-SOSIP trimer. (<b>c</b>) Distances between K169 and R166 side chains in the PGT145-bound SOSIP trimer structure (PDB ID 5V8L), with the PGT145 CDRH3 contact region shown in pink. (<b>d</b>) Tilted side view of GLA-SOSIP trimer revealing extra density (yellow) corresponding to an inter-gp120 protomer cross-link between K155 and R178. (<b>e</b>) Tilted side view of GLA-SOSIP trimer revealing cross-linking (yellow) between gp120 residues K227, K229 and K485, pr...