p38b and JAK-STAT signaling protect against Invertebrate iridescent virus 6 infection in <i>Drosophila</i> - Fig 5

<p><b><i>p38b</i> is required for <i>turandot</i> induction and survival A)</b> Mutant strains for the three p38 homologs, <i>p38a</i>^<i>MPK1</i>, <i>p38b</i>^<i>ex9</i>, or <i>p38c</i>^<i>1A1</i>, were infected with IIV-6 or injected with PBS, and <i>TotA</i> expression was measured by qRT-PCR 24 hours post-infection. Control genotypes included <i>w</i>^<i>1118</i> and <i>p38b</i>^<i>ex9</i>/<i>CyO</i> heterozygotes. The results of 3–5 biologically independent replicates are shown. Black bars indicate the mean and error bars represents standard deviation. Statistical analysis, comparing <i>TotA</i> levels in the virus infected control to mutant strains, was determined by two-way ANOVA with Sidak’s correction for multiple comparisons (***p = 0.0004, ** p = 0.0045) <b>B)</b> Kaplan-Meier curves showing survival of homozygous (<i>p38b</i>^<i>ex9</i>, red lines) or heterozygous (<i>p38b</i>^<i>ex9</i><i>/CyO</i>, orange lines) <i>p38b</i> mutant flies following IIV-6 infection (solid lines) or PBS-injected controls (dotted lines) compared to control (<i>w</i>^<i>1118</i>, black lines) flies. <b>C)</b> Kaplan-Meier curves showing survival of <i>p38a</i> mutant flies (red lines) following IIV-6 infection (solid lines) or PBS-injected controls (dotted lines) compared to control (<i>w</i>^<i>1118</i>, black lines) flies. <b>D)</b> Kaplan-Meier curves showing survival of <i>p38b</i>^<i>RNAi</i> expressing (blue lines) or <b>E)</b> <i>p38a</i>^<i>RNAi</i> expressing flies (purple lines) following IIV-6 infection (solid lines) or PBS-injected controls (dotted lines). <i>UAS-p38a</i>^<i>RNAi</i> and <i>UAS-p38b</i>^<i>RNAi</i> flies were crossed to <i>tubulin-GAL4</i> for ubiquitous knock-down, while the control was generated by <i>w</i>^<i>1118</i> crossed to <i>tubulin-GAL4</i> (black lines). Statistical significance was determined by Log-rank (Mantel-Cox) test (**** p <0.0001; ns, not significant). A.U., Arbitrary Units. <b>F)</b> Viral loads as determined by QPCR for <i>p38b</i>^<i>ex9</i> and <i>p38b</i>^<i>ex9</i><i>/CyO</i> heterozygous siblings. Each data point represents 5 flies. Error bars indicate standard error, and black lines indicate the mean. A.U., arbitrary units. Statistics were determined using two-way ANOVA. ns, not significant.