MOESM5 of Promiscuous activities of heterologous enzymes lead to unintended metabolic rerouting in Saccharomyces cerevisiae engineered to assimilate various sugars from renewable biomass

Additional file 5: Figure S3. TLC analysis of the reaction products obtained from in vitro reactions with various substrates, namely galactose (Gal), cellobiose (CB), xylose (Xyl), and glucose (Glc) using a crude enzyme extract of S. cerevisiae D452-2 or EJ4 to measure transglycosylase activity. The enzyme mixture containing 1 mg/mL crude cell-free lysate enzymes obtained from S. cerevisiae D452-2 or EJ4, 2 mg/mL one of the substrates, and 20 mM Tris–HCl buffer (pH 7.0) was incubated at 30 °C and 200 rpm for 12 h. The products of the enzymatic reactions involving crude cell-free lysate enzymes of (A) S. cerevisiae D452-2 or (B) EJ4 were analyzed by TLC