WCR-R larvae and beetles are resistant to DvSnf7 dsRNA and inheritance of DvSnf7 dsRNA resistance is recessive.

<p>(<b>a</b>) Concentration–response curves of WCR-S, WCR-R neonates and F1 neonates from WCR-R and WCR-S reciprocal crosses. Each cross was bulk-mated and consisted of 80 males and 80 females. Each population was assayed using 5–6 concentrations (<i>n</i> = 105–254). (<b>b</b>) Percent beetle mortality from WCR-R and WCR-S when exposed to artificial diet overlaid with DvSnf7 dsRNA (1000 ng /cm²) or water (0 ng /cm²). Assay was conducted one time with three replications, each consisting of 10 beetles (<i>n</i> for all treatments = 30). (<b>c</b>) DvSnf7 transcript levels determined using RT-qPCR in beetles fed for 72 hrs on artificial diet overlaid with DvSnf7 dsRNA or water (<i>n</i> = 7–8). (<b>d</b>) Percent beetle mortality from WCR-R and WCR-S when injected with 25 ng of either DvSnf7 or GFP dsRNA. Mortality was recorded 10 d after injection. Assay was conducted two times each with three replications (<i>n</i> = 30–48). (<b>e</b>) DvSnf7 transcript levels determined using RT-qPCR in beetles injected with 25 ng of DvSnf7 or GFP dsRNA. After injections beetles were allowed to feed on untreated artificial diet for 72 h and collected for transcript analysis (<i>n</i> = 3–8). (c to e) Means ± SEM. **<i>p</i> < 0.0001, *<i>p</i> = 0.042.